Bosphore HPV Genotyping LR HR Kit v1

Bosphore® HPV HR-LR-Genotyping Kit v1 detects and characterizes the genotype of Human Papilloma Virus in human biological samples (swab, tissues, biopsy specimen, serum, plasma), encompassing high risk (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) and low risk (6, 11, 42, 61, 70) groups of HPV genotypes. Fluorescence detection is accomplished using the FAM, HEX, Texas RED and Cy5 filters.

 

The genotypes 16, 18, 31 and internal control are detected in PCR Master Mix 1; the genotypes 51, 56, 58 and internal control are detected in PCR Master Mix 2, the genotypes 35, 45, 59 and internal control are detected in PCR Master Mix 3, the genotypes, 39, 66, 68 and internal control are detected in PCR Master Mix 4 and the genotypes 33, 52 and internal control are detected in PCR Master Mix 5, the genotypes 6, 11, 42 and internal control are detected in PCR Master Mix 6, the genotypes 61, 70 and internal control are detected in PCR Master Mix 7 with using labeled probes. Seven PCR master mixes are used in seven separate wells/reactions.

 

An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.