Food Safety Testing

Foodborne pathogens have always been a major concern for public health. Surveillance and identification of the source of the outbreak can be a challenge. The pathogens originating from farm animal or wildlife animal excrements first contaminate the groundwater. The contaminated source in turn contaminates every process and environment it is used. It is of utmost importance that the source must be identified as quickly as possible to control and stop the outbreak.

Bosphore Food Safety Testing Kits serve as sensitive, accurate and reliable tools for samples collected to help protect public health.

Features

Ready to use

PCR inhibition resistant

LOD up to 13 copies

Process control

One-Step QPCR solution

Time to results under 90 min

Workflow

Technical Specifications

The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.

Thermal Protocol Single thermal protocol for all parameters
Sample Types Processed and unprocessed food, feed, seed samples
Shelf Life 18 Months
Channels FAM, HEX, Texas Red, Cy5
Shipping / Storage (-90°C)-(-20°C) / -20°C

Food Safety Testing Kits

Bosphore Hepatitis A Detection Kit v2 detects hepatitis A RNA from fruit and vegetable samples, sea food, meat, poultry and dairy products. Hepatitis A genome is amplified and fluorescence detection is accomplished using FAM filter. The analytical detection limit for Bosphore Hepatitis A Detection Kit v2 was found to be 21 copies/reaction.

A process control has been integrated into the kit to control extraction efficiency. The amplification data of the process control is detected with the Cy5 filter. Also, positive control (external control-EC) has been integrated to the kit to control RT-PCR inhibition and to act as an RT-PCR positive control.

Bosphore Norovirus Detection Kit v2 detects norovirus GI and GII RNA from fruit and vegetable samples, sea food, meat, poultry and dairy products. Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters. Norovirus genogroup GI genome is amplified and fluorescence detection is accomplished using FAM filter and norovirus genogroup GII genome is amplified and fluorescence detection is accomplished using HEX filter. Analytical detection limit for Bosphore Norovirus Detection Kit v2 was found to be 13 copies/reaction for norovirus GI and 20 copies/reaction for norovirus GII.

A process control has been integrated into the kit to control extraction efficiency. Amplification data of the process control is detected with the Cy5 filter. Also, positive control (external control-EC) has been integrated to the kit to control RT-PCR inhibition and to act as an RT-PCR positive control.

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