Real-Time PCR Kitleri
Celiac
Celiac disease (CD) is a chronic gluten intolerance that occurs in genetically predisposed individuals. In sensitive individuals, the ingestion of gluten determines chronic inflammation of the small intestinal mucosa that results in villous atrophy, crypt hyperplasia, and lymphocyte infiltration, leading to nutrient malabsorption.
Specific variants of the HLA-DQA1 and HLA-DQB1 genes play a crucial role in CD development. While traditional diagnostic methods like serological and histological methods may be nonspecific or inadequate for some cases, Real-Time PCR Kits can help identify high-risk alleles and assess the risk of developing CD.
Features
Workflow
Technical Specifications
| Sample Types | Human whole blood and buccal swab |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas RED, Cy5 |
| Shipping/Storage | (-90°C)-(-20°C) / -20°C |
Celiac Kits
Bosphore Celiac HLA DQ2/DQ8 Detection Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the DQA1*02, DQA1*03, DQA1*05, DQB1*02 and DQB1*03:02 alleles in Human Leukocyte Antigen (HLA) DQA1 and DQB1 genes from human whole blood and buccal swab samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM | HEX | Texas RED | Cy5 (Gene) |
| PCR Master Mix 1 | DQA1*02 | DQB1*02 | DQA1*05 | Internal Control (GAPDH) |
| PCR Master Mix 2 | DQA1*03 | DQB1*03:02 | – | Internal Control (GAPDH) |
Internal control based on the detection of the endogenous nucleic acid sequence of the GAPDH gene present in the human genome has been integrated into the kit to demonstrate the proper functioning of the PCR reaction in cases where these alleles are not present, and to check for sampling, extraction procedure, PCR inhibition, or application problems. The amplification data of the internal control is detected using the Cy5 filters.
Certain variants of the HLA-DQA1 and HLA-DQB1 genes provide the main susceptibility molecules for Celiac disease. These molecules include HLA-DQ2.2, HLA-DQ2.5, and HLA-DQ8, which are collectively present in almost all (99%) patients with Celiac disease. Bosphore Celiac HLA DQ2/DQ8 Detection Kit v1 detects the following alleles:
- HLA-DQA1*02 and HLA-DQB1*02, which encode the HLA-DQ2.2 molecule,
- HLA-DQA1*05 and HLA-DQB1*02, which encode the HLA-DQ2.5 molecule, and
- HLA-DQA1*03 and HLA-DQB1*03:02, which encode the HLA-DQ8 molecule.
Although these molecules, especially HLA-DQ2.5, confer a substantial relative risk for Celiac disease, the absolute risk remains low, as most individuals carrying one or more of these genes may not develop Celiac disease. On the other hand, the absence of these specific allele combinations effectively rules out the disease. Bosphore Celiac HLA DQ2/DQ8 Detection Kit v1 is sufficient to exclude Celiac disease in such cases.
