Real-Time PCR Kits
Lactose Intolerance
Lactose intolerance is a chronic condition caused by a deficiency of the lactase enzyme, which is necessary for digesting lactose. In individuals with lactase persistence, the enzyme remains active into adulthood, allowing them to digest lactose without issues. However, those with lactase non-persistence experience fermentation of undigested lactose in the colon, leading to symptoms such as bloating, diarrhea, abdominal pain, and gas, with severe cases potentially causing nutrient malabsorption.
The LCT gene regulates lactase production, with 13910C/T and 22018G/A polymorphisms being associated with lactase persistence. This trait is more prevalent in populations of European descent, whereas lactase non-persistence is more common among Asian, African, and Native American populations. Diagnosing lactose intolerance can be challenging, as traditional methods like blood glucose tests, serological tests, and hydrogen breath tests may yield false negatives due to variations in symptom presentation and diagnostic accuracy. Genetic testing can estimate the risk of having lactose intolerance based on the detected combination of alleles.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Sample Types | Human whole blood and buccal swab |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas RED, Cy5 |
| Shipping/Storage | (-90°C)-(-20°C) / -20°C |
Lactose Intolerance Kits
Bosphore Lactose Intolerance Screening Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 13910C/T and 22018G/A alleles in the LCT (Lactase) gene from human whole blood and buccal swab samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix | 13910C | 13910T | 22018G | 22018A |
Certain variants in the LCT (Lactase) gene are associated with lactose intolerance, affecting the ability to digest lactose in adulthood. The 13910C/T and 22018G/A polymorphisms in the regulatory region of the LCT gene are key markers for lactase persistence or deficiency.
Bosphore Lactose Intolerance Screening Kit v1 detects the following alleles:
- 13910C/T polymorphism: Located upstream of the LCT gene, associated with lactase persistence (T allele) or lactase non-persistence (C allele).
- 22018G/A polymorphism: Another regulatory variant linked to lactase expression levels. Located upstream of the LCT gene, associated with lactase persistence (A allele) or lactase non-persistence (G allele).
Bosphore Lactose Intolerance Screening Kit v1 is a genetic diagnostic test that identifies key polymorphisms associated with lactose intolerance. However, lactose intolerance can also be influenced by epigenetic modifications and environmental factors, such as gut microbiota, diet, and intestinal health.
