Real-Time PCR Kits
Polyomavirus
BK and JC viruses (BKV and JCV) are polyomaviruses belonging to Polyomaviridae family. Most of the primary polyomavirus infections occur during childhood. Serological studies suggest that more than 70% of adults possess antibodies against BK or JC virus. There are serological evidences suggesting the reactivation of the JCV and BKV viruses during the pregnancy period of 5-10% of the women. The diagnosis and monitoring of the BK and JCV infections are managed through histological and cytological tests with the help of high electron and fluorescence microscopy. Also the serological methods (haemagglutination inhibition test, ELISA,RIAs ) based on the detection of antibodies against polyoma viruses are used widespread. Early diagnosis of BK and JCV reactivation is paramount for prevention of the possible severe diseases that even positively impacts organ survival.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Serum and plasma |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Polyomavirus Kits
Bosphore BKV Quantification Kit v1 detects and quantitates all genotypes of BK virus DNA in human serum, plasma and urine samples. The analytic sensitivity is 75 copies/ml. A region within the large T-antigen encoding gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with the HEX filter. The internal control is added either in extraction or PCR step.
| FAM | HEX |
| BKV | Internal Control |
Bosphore JCV Quantification Kit v1 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore JCV Quantification Kit v1 detects and quantitates JC virus DNA in human serum or plasma sample. A region within the large T-antigene encoding gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added either in extraction or PCR step.
Bosphore JCV-BKV Detection Kit v2 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the VP2 genes of John Cunningham virus (JCV) and BK virus (BKV) from serum, plasma, urine, tissue/biopsy, BAL, amniotic fluid and CSF samples. Fluorescence detection is performed using FAM, HEX, and Cy5 filters. JCV DNA is amplified and fluorescence detection is performed using the FAM filter and BKV DNA is amplified and fluorescence detection is performed using the HEX filter.
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or the PCR step.
