Real-Time PCR Kits
SARS-CoV-2
SARS-CoV-2, or Severe Acute Respiratory Syndrome Coronavirus 2, is a member of the large coronavirus family. It is the causative agent of COVID-19, the respiratory illness responsible for the global COVID-19 pandemic. The virus was previously referred to as the 2019 novel coronavirus (2019-nCoV) and human coronavirus 2019 (HCoV-19 or hCoV-19).
SARS-CoV-2 is a single-stranded RNA virus that is highly contagious in humans. Symptoms of infection can include fever, cough, and difficulty breathing, ranging from mild to severe illness and, in some cases, leading to death.
SARS-CoV-2 primarily spreads through respiratory droplets and aerosols when an infected person talks, coughs, sneezes or breathes. It can also spread via contact with contaminated surfaces, although this mode is less common. Close contact with infected individuals increases the risk of transmission, and asymptomatic carriers can also spread the virus.
SARS-CoV-2 infection is most commonly diagnosed using molecular tests like Real-Time PCR, which detects the virus’s genetic material. With Bosphore SARS-CoV-2 Real-Time PCR Kits, in-vitro diagnosis of SARS-CoV-2 is easy, accurate and fast.
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Respiratory samples such as nasopharyngeal swabs, oropharyngeal swabs, saliva, and sputum |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5, Quasar 705, ROX |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
SARS-CoV-2 Kits
Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the SARS-CoV-2, RSV A/B, Influenza A/B, Enterovirus, Human Metapneumovirus A/B, Adenovirus, Human Parainfluenza viruses 1/2/3/4, Rhinovirus, Mycoplasma pneumoniae, and Legionella pneumophila from human respiratory samples including nasopharyngeal, oropharyngeal swabs, and saliva. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| FAM | HEX | Texas RED | Cy5 | |
| PCR Master Mix 1 | SARS-CoV-2 | RSV A/B | Internal Control | Influenza A/B |
| PCR Master Mix 2 | Enterovirus | Human Metapneumovirus A/B | Internal Control | Adenovirus |
| PCR Master Mix 3 | Human Parainfluenza Viruses 1/2/3/4 | Rhinovirus | Legionella pneumophila | Mycoplasma pneumoniae |
An internal control based on the detection of human endogenous nucleic acid sequence present in the human genome has been employed to check DNA/RNA extraction, PCR inhibition, or application problems. The kit also contains positive controls.
Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v3 Real-Time PCR kit for in vitro diagnostics that detects and characterizes the specified gene regions of the following pathogens from human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix 1 | SARS-CoV-2 | RSV A/B | Influenza A | Influenza B |
| PCR Master Mix 2 | Enterovirus | Human metapneumovirus A/B | Internal Control | Adenovirus |
| PCR Master Mix 3 | Human parainfluenza virus type 1/2/3/4 | Rhinovirus | Legionella pneumophila | Mycoplasma pneumoniae |
An internal control based on detecting human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check nucleic acid extraction, PCR inhibition, and sampling or application errors. Amplification data of the internal control is detected with the Texas RED filter for PCR Master Mix 2.
Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v4 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM | HEX
|
Texas RED
|
Cy5
|
| PCR Master Mix
1 |
Pandemic H1N1
|
Parechovirus
|
– | – |
| PCR Master Mix
2 |
Parainfluenza 1
|
Rhinovirus
|
Exogenous
Internal Control
|
Mycoplasma pneumoniae |
| PCR Master Mix
3 |
Enterovirus
|
Coronavirus NL63
|
Coronavirus 229E
|
Coronavirus HKU1
|
| PCR Master Mix
4 |
Parainfluenza 4 | Endogenous
Internal Control |
Parainfluenza 2
|
Parainfluenza 3
|
| PCR Master Mix
5 |
Coronavirus OC43
|
Human metapneumovirus A/B | Bocavirus
|
Adenovirus |
| PCR Master Mix
6 |
SARS-CoV-2
|
RSV A/B
|
Influenza A
|
Influenza B |
An exogenous internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition, and application mistakes. The amplification data of the exogenous internal control is detected with the Texas RED filter in PCR Master Mix 2. The internal control can be added either during DNA/RNA extraction or the PCR step.
An endogenous internal control based on the detection with the HEX filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check sampling, extraction, and application errors. The endogenous internal control amplification data is detected with the HEX filter in PCR Master Mix 4.
Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v5 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, ROX, and Cy5 filters.
| Component | FAM
|
HEX
|
ROX
|
Cy5
|
| PCR Master Mix 1 | Adenovirus
|
RSV A/B
|
Influenza A
|
Influenza B
|
| PCR Master Mix 2 | Enterovirus
|
Human metapneumovirus A/B
|
Bocavirus
|
SARS-CoV-2
|
| PCR Master Mix 3 | Pandemic H1N1
|
Rhinovirus
|
Mycoplasma pneumoniae | Coronavirus OC43/HKU1
|
| PCR Master Mix 4 | Human parainfluenza virus type 1/2/3/4 | Parechovirus
|
Endogenous Internal Control | Coronavirus NL63/229E |
An endogenous internal control based on the detection with the ROX filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check sampling, extraction, PCR inhibition, and application errors. The endogenous internal control amplification data is detected with the ROX filter in PCR Master Mix 4.
Bosphore SARS-CoV-2/Flu/RSV Panel Kit is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 2019-nCoV (which was later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and Influenza A&B in a single tube from human respiratory samples including nasopharyngeal, oropharyngeal swabs, sputum and saliva. Fluorescence detection uses FAM, HEX, Texas RED, and Cy5 filters. SARS-CoV-2 RNA is amplified, and fluorescence detection is performed using the FAM filter, RSV A&B RNA is amplified, and fluorescence detection is performed using the HEX filter, and Influenza A&B RNA is amplified, and fluorescence detection is performed using the Cy5 filter.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix | SARS-CoV-2 | RSV A&B | Internal Control | Influenza A&B |
An internal control based on detecting human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check RNA extraction, PCR inhibition, sampling, or application errors. Internal control is detected by the Texas RED filter.
Bosphore SARS-CoV-2/Flu/RSV Panel Kit v2 detects and discriminates 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and influenza A, influenza B in human respiratory samples including nasopharyngeal, oropharyngeal swabs and oral fluids. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.
SARS-CoV-2 is detected by three regions of the virus in a single reaction with Master Mix 1: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the Orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. RSV A&B, influenza A and influenza B are detected and discriminated with Master Mix 2.
| Component | FAM | HEX | Texas Red | Cy5 |
| PCR Master Mix 1 | Orf1ab
(SARS-CoV-2) |
Internal Control (RNase P) | N gene
(SARS-CoV-2) |
E gene
(SARS-CoV-2) |
| PCFR Master Mix 2 | Influenza A | RSV A&B | – | Influenza B |
Bosphore SARS-CoV-2/Flu/RSV Panel Kit v6 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 2019-nCoV (which is later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and Influenza A&B from human respiratory samples including nasopharyngeal, oropharyngeal swabs, sputum and saliva.
Fluorescence detection is accomplished using FAM, HEX, Texas RED, Cy5, and Quasar 705 filters.
| Component | FAM | HEX | Texas RED | Cy5
|
Quasar 705 |
| PCR Master Mix | SARS-CoV-2 | RSV A&B | Internal Control | Influenza B | Influenza A |
An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed in order to check RNA extraction, PCR inhibition, and sampling or application errors. Internal control is detected by the Texas RED channel.
Bosphore Novel Coronavirus (2019-nCoV) Detection Kit is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.
Bosphore Novel Coronavirus (2019-nCoV) Detection Kit detects and characterizes 2019-nCoV in human respiratory samples.
Fluorescence detection is accomplished using FAM and HEX filters. 2019-nCoV is detected by two regions of the virus in two separate reactions: E gene is used for screening purpose, where 2019-nCoV and also the closely related coronaviruses are detected, and the Orf1ab target region is used to discriminate 2019-nCoV specifically. In the first PCR tube with PCR Master Mix 1, 2019-nCoV target region is amplified and fluorescence detection is accomplished using FAM filter. In the second PCR tube with PCR Master Mix 2, 2019-nCoV screening region (E gene) is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check RNA extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with HEX filter in each tube. The internal control can be added either during RNA extraction or PCR step.
| Component | FAM | HEX |
| PCR Master Mix 1 | Orf1ab | Internal Control |
| PCR Master Mix 2 | E gene | Internal Control |
Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v2 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.
Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v2 detects and characterizes 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters. 2019-nCoV is detected by two regions of the virus in two separate reactions: E gene is used for screening purpose, where 2019-nCoV and also the closely related coronaviruses are detected, and the Orf1ab target region is used to discriminate 2019-nCoV specifically. In PCR Master Mix, 2019-nCoV target region is amplified and fluorescence detection is accomplished using FAM filter, 2019-nCoV screening region (E gene) is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check RNA extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with HEX filter.
The internal control can be added either during RNA extraction or PCR step.
In order to tackle the existing global shortage on extraction kits under these extra-ordinary circumstances, we have developed an alternative, fast-extraction, which does not require a separate extraction but only a pre-treatment that takes less than 10 minutes. This method helps the institutions and medical workers save precious time by eliminating the need for extraction for diagnosis. Bosphore EX-Tract Dry Swab RNA Solution has been optimized to perform only with Bosphore Novel Coronavirus (2019-nCoV) Detection Kits.
| FAM | HEX | Cy5 |
| Orf1ab | Internal Control | E gene |
Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v3 detects and characterizes 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum.
Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. SARS-CoV-2 is detected by three regions of the virus in a single reaction: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, and the Orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically.
An internal control has been integrated into the kit in order to check RNA extraction, PCR inhibition and application errors. The internal control can be added either during RNA extraction or PCR step.
In order to tackle the existing global shortage on extraction kits under these extra-ordinary circumstances, we have developed an alternative, fast-extraction, which does not require a separate extraction but only a pre-treatment that takes less than 10 minutes. This method helps the institutions and medical workers save precious time by eliminating the need for extraction for diagnosis. Bosphore EX-Tract Dry Swab RNA Solution has been optimized to perform only with Bosphore Novel Coronavirus (2019-nCoV) Detection Kits.
| FAM | HEX | Texas Red | Cy5 |
| Orf1ab | Internal Control | N gene | E gene |
Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v4 detects and characterizes 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum.
Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. SARS-CoV-2 is detected by three regions of the virus in a single reaction: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically.
An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.
| FAM | HEX | Texas Red | Cy5 |
| Orf1ab | Internal Control
(Human RNase P gene) |
N gene | E gene |
Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v7 detects and characterizes 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum.
Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. SARS-CoV-2 is detected by three regions of the virus in a single reaction: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically.
An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.
| FAM | HEX | Texas RED | Cy5 |
| Orf1ab | Internal Control
(Human RNase P gene) |
N gene | E gene |
Bosphore Coronavirus Panel Kit v1 detects and characterizes Coronavirus 229E, Coronavirus HKU1, Coronavirus OC43 and Coronavirus NL63 in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
In the first PCR tube with PCR Master Mix 1, Coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas Red filter, Coronavirus HKU1 genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the second PCR tube with PCR Master Mix 2, Coronavirus OC43 genome is amplified and fluorescence detection is accomplished using the FAM filter, Coronavirus NL63 genome is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the Internal Control is detected with the HEX filter in PCR Master Mix 1, Texas Red filter in PCR Master Mix 2. The Internal Control can be added either during DNA extraction or PCR step.
