Magrev Kits are based on binding and elution of nucleic acids to and from magnetic beads coated with silica.
Nucleic acid extraction is the first step to be applied for all molecular biology based downstream applications. Numerous molecular genetic tests and experiments that are commonly used are performed only after the extraction of DNA or RNA of targeted organism. DNA/RNA isolation is generally composed of cell membrane and cell wall degradation, inactivation of nuclease activity, removal of proteins, concentrating the nucleic acids and appropriate storage of the extracted DNA/RNA. Extraction is formed from 4 main steps named as; lysis (release of DNA by degrading the cells and the structures bound to membrane, separation and denaturation of the proteins from DNA/RNA), binding (binding of DNA/RNA on a surface), washing (removal of unwanted contaminants such as salt, protein, etc.) and elution (recovery of DNA/RNA from the surface bound).