Real-Time PCR Kits
Rash Panel
Human enteroviruses are a common cause of infections affecting millions globally each year. These viruses are influenced by seasonal and climatic conditions. While most infections are asymptomatic or mild, outbreaks are more common in tropical regions during warm and rainy seasons. Enteroviruses are primarily transmitted via the fecal-oral route, though some exceptions, such as those spread by respiratory secretions or tears, can be transmitted via fingers and contaminated surfaces (fomites).
Parechoviruses typically cause mild respiratory and gastrointestinal symptoms in neonates and young infants. However, in some cases, they can lead to severe conditions such as pneumonia, encephalitis, stroke, or myocarditis, particularly in young children.
Infections caused by the Herpesviridae family of viruses present with diverse clinical symptoms. Most human herpesviruses are transmitted through physical contact or airborne particles.
Parvovirus B19, a member of the Parvoviridae family, is a small DNA virus that infects humans. It is responsible for erythema infectiosum, arthritis, and joint pain.
Bosphore Rash Panel Kits provide a comprehensive approach to diagnosing rashes associated with these viral infections, aiding in prompt identification and diagnosis.
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Vesicle swabs, vesicle fluids, tissue/biopsy specimen, throat swab, sputum, serum, plasma, and CSF. |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Rash Panel Kits
Bosphore Rash-Fever Panel Kit v1 detects and characterizes parvovirus B19, human herpes virus 6 (HHV-6), human herpes virus 7 (HHV-7), enterovirus and parechovirus in human biological samples including tissue/biopsy specimen, throat swab, sputum, serum, plasma, and CSF. The test is accomplished in two PCR tubes. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. The internal control can be added either during nucleic acid extraction or PCR step.
| FAM | HEX | Texas Red | Cy5 | |
| Tube 1 | HHV-6 | Parvovirus B19 | Internal Control | HHV-7 |
| Tube 2 | Enterovirus | Parechovirus | Internal Control | – |
Bosphore Vesicular Rash Panel Kit is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Vesicular Rash Panel Kit detects and characterizes herpes simplex virus 1, herpes simplex virus 2 and varicella-zoster virus DNA in human biological samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters. Herpes simplex virus 1 is amplified and fluorescence detection is accomplished using the FAM filter, herpes simplex virus 2 is amplified and fluorescence detection is accomplished using the Cy5 filter and varicella-zoster virus is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix | HSV-1 | VZV | Internal Control | HSV-2 |
