Respiratory Pathogens Panel

Bosphore ADEP Panel Kit detects and characterizes enterovirus, parechovirus, and adenovirus in human biological samples including tissue/biopsy specimen, BAL, nasopharyngeal swab and aspirate, tracheal aspirate, sputum and CSF. The test is accomplished in a single tube qualitatively, encompassing all the subtypes of enterovirus, parechovirus, and adenovirus. A region in enterovirus genome is amplified and fluorescence detection is accomplished using the FAM filter, a region in parechovirus genome is amplified and fluorescence detection is accomplished using the HEX filter, and a region in adenovirus genome is amplified and fluorescence detection is accomplished using the Cy5 filter.

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step. The amplification data of the internal control is detected with the Texas Red filter.

Bosphore Influenza A-B Detection Kit v1 detects Influenza A RNA and Influenza B RNA in human serum, plasma, respiratory samples, and nasopharyngeal specimens. The analytic sensitivity for Influenza A is 15 copies/reaction and for Influenza B is 2 copies/reaction. Influenza A virus RNA is amplified and detected with FAM filter and Influenza B virus RNA is amplified and detected with HEX filter. 

The endogenous internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during the nucleic acid extraction step or PCR step. 

Bosphore Influenza A-B-C Detection Panel Kit v1 detects and characterizes Influenza A, Influenza B, and Influenza C in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters. Influenza A genome is amplified, and fluorescence detection is accomplished using FAM filter. Influenza B genome is amplified, and fluorescence detection is accomplished using HEX filter. Influenza C is amplified, and fluorescence detection is accomplished using Cy5 filter. An internal control has been integrated into the kit to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.

Bosphore Influenza Typing Kit v1 detects and discriminates against Influenza A virus, Influenza B virus, pandemic H1N1 influenza A virus (novel influenza A H1N1/2009 H1N1 virus), and Influenza A H3 virus in human respiratory samples including nasopharyngeal and oropharyngeal swabs. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters. 

An internal control for master mix 1, has been integrated into the kit to check RNA extraction, PCR inhibition and application errors. The internal control is added during the nucleic acid extraction step or PCR step.  

Internal control for master mix 2, based on the detection of human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check RNA extraction, PCR inhibition and sampling or application errors. For both master mix 1 and 2, internal control is detected by the Cy5 channel. 

Bosphore Influenza Typing Kit v2 detects and discriminates pandemic H1N1 influenza A virus (novel influenza A H1N1/2009 H1N1 virus), Influenza A H3 virus, Influenza A H5 virus, Influenza A H7 virus, in human respiratory samples including nasopharyngeal and oropharyngeal swabs. Fluorescence detection is accomplished using FAM, HEX, Texas RED and Cy5 filters.  

An internal control for Master Mix 1, based on the detection of human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check RNA extraction, PCR inhibition and sampling or application errors.  For PCR Master Mix 1, internal control is detected by Cy5 channel. 

An internal control for Master Mix 2, has been integrated into the kit to check RNA extraction, PCR inhibition and application errors. The internal control is added during the nucleic acid extraction step or PCR step. For PCR Master Mix 2, internal control is detected by HEX channel. 

Bosphore Bacterial Pneumonia CAP Bundle Kit detects and discriminates Chlamydophila pneumoniae, Legionella pneumophila/longbeachae, Mycoplasma pneumoniae, H. influenzae, M. catarrhalis, S. pneumoniae, and S. aureus bacteria in human biological samples including throat swab, nasopharyngeal swab, nasopharyngeal aspirate, tracheal aspirate, bronchoalveolar lavage, sputum, biopsy (tissue) samples and culture in three PCR tubes. Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters.  

An internal control has been integrated into the kit to check DNA extraction, PCR inhibition, and application mistakes. The internal control can be added either during the DNA extraction or PCR step. 

Bosphore Bacterial Pneumonia CAP Bundle Kit is composed of Bosphore Atypical CAP Panel Kit and Bosphore Bacterial Pneumonia Mini Panel Kit. 

Bosphore Respiratory Pathogens Panel Kit v5 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the gene regions of the pathogens listed below from human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.  

Internal Control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the Internal Control is detected with the Texas RED and Cy5 filters. The Internal Control can be added either during DNA/RNA extraction or the PCR step. 

Bosphore Respiratory Pathogens Panel Kit v6 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. 

Internal Control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the Internal Control is detected by the filters specified in the table. The Internal Control can be added either during DNA/RNA extraction or the PCR step. 

Bosphore Respiratory Pathogens Panel Kit v7 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nose swab, nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.

An exogenous internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition, and application mistakes. The amplification data of the exogenous internal control is detected with the Texas RED filter in PCR Master Mix 2, 4, 5, and 6 and; the Cy5 filter in PCR Master Mix 1 and 3. The internal control can be added either during DNA extraction or the PCR step.

An endogenous internal control based on the detection with the HEX filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check extraction, PCR inhibition, and sampling or application errors. The endogenous internal control amplification data is detected with the HEX filter in PCR Master Mix 7.

Bosphore Respiratory Pathogens Panel Kit v8 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. 

An exogenous internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition, and application mistakes. The amplification data of the exogenous internal control is detected with the Texas RED filter in PCR Master Mix 1, 2, 5, 6, and 7; the Cy5 filter in PCR Master Mix 3 and 4; and the FAM filter in PCR Master Mix 8. The internal control can be added either during DNA/RNA extraction or the PCR step. 

An endogenous internal control based on the detection with the Cy5 filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check extraction, PCR inhibition, and sampling or application errors. The endogenous internal control amplification data is detected with the Cy5 filter in PCR Master Mix 9. 

Bosphore Respiratory Pathogens Panel Kit v9 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.  

 An endogenous internal control based on the detection of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check sampling, DNA/RNA extraction, PCR inhibition, sampling, or application problems. The amplification data of the internal control is detected with the Texas RED filter of PCR Master Mix 1.  

*Available in Strip format 

Bosphore Respiratory Pathogens Panel Kit v10 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swabs and nasopharyngeal swabs. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.  

 An exogenous internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the exogenous internal control is detected with the Texas RED filter of PCR Master Mix 1. Internal control can be added during the DNA/RNA extraction step.  

An endogenous internal control based on the detection with the Cy5 filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check RNA extraction, PCR inhibition, sampling, or application errors. The amplification data of the endogenous internal control is detected with the Cy5 filter in PCR Master Mix 8.  

*Available in Strip format 

Bosphore Respiratory Pathogens Panel Kit v2s is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.  

 An exogenous internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the exogenous internal control is detected with the Texas RED filter of PCR Master Mix 1. The internal control can be added during the DNA/RNA extraction step.  

An endogenous internal control based on the detection with the Cy5 filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed in order to check DNA/RNA extraction, PCR inhibition, sampling, or application errors. The amplification data of the endogenous internal control is detected with the Cy5 filter in PCR Master Mix 8.  

Bosphore Respiratory Pathogens Maxi Panel detects and characterizes adenovirus, bocavirus, Bordetella pertussis, coronavirus 229E, coronavirus OC43, coronavirus NL63, coronavirus HKU1, Chlamydia pneumoniae, enterovirus, Haemophilus influenzae type B, influenza A, influenza B, influenza C, Klebsiella pneumoniae, Legionella pneumophila, Legionella pneumophila / longbeachae, Mycoplasma pneumoniae, Moraxella catarrhalis, metapneumovirus, RSV A/B, pandemic H1N1 influenza A, parainfluenza 1, parainfluenza 2, parainfluenza 3, parainfluenza 4, parechovirus, Pneumocystis jirovecii, rhinovirus, seasonal H1N1 influenza A, Salmonella enterica, Staphylococcus aureus, and Streptococcus pneumoniae  in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.

An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with HEX, Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.

Bosphore Respiratory Pathogens Mini Panel Kit detects and characterizes parainfluenza 1-2-3-4, enterovirus, metapneumovirus, influenza A-B, pandemic H1N1, RSV A/B, bocavirus, rhinovirus, coronavirus 229E-HKU1-NL63-OC43, adenovirus, H. influenza type B, parechovirus, Staphylococcus aureus, Streptococcus pneumoniae and Mycoplasma pneumoniae in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.

An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with HEX, Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.

Bosphore Respiratory Pathogens Mini Panel Bundle Kit detects and characterizes parainfluenza 1, parainfluenza 2, parainfluenza 3, parainfluenza 4, enterovirus, metapneumovirus, influenza A, influenza B, pandemic H1N1, RSV A/B, bocavirus, rhinovirus, coronavirus OC43, coronavirus NL63, coronavirus HKU1, coronavirus 229E, adenovirus, H. influenza type B, parechovirus, Staphylococcus aureus, Streptococcus pneumoniae, Mycoplasma pneumoniae, Legionella pneumophila / longbeachae, Chlamydophila pneumoniae in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.

An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with HEX, Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.

Bosphore Respiratory Viral Basic Panel Kit detects and characterizes adenovirus, enterovirus, Legionella pneumophila, metapneumovirus, Mycoplasma pneumoniae, rhinovirus, and parainfluenza viruses (1, 2, 3, 4) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.

An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.

Bosphore Coronavirus Panel Kit v1 detects and characterizes Coronavirus 229E, Coronavirus HKU1, Coronavirus OC43 and Coronavirus NL63 in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.

In the first PCR tube with PCR Master Mix 1, Coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas Red filter, Coronavirus HKU1 genome is amplified and fluorescence detection is accomplished using the Cy5 filter.

In the second PCR tube with PCR Master Mix 2, Coronavirus OC43 genome is amplified and fluorescence detection is accomplished using the FAM filter, Coronavirus NL63 genome is amplified and fluorescence detection is accomplished using the HEX filter.

An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the Internal Control is detected with the HEX filter in PCR Master Mix 1, Texas Red filter in PCR Master Mix 2. The Internal Control can be added either during DNA extraction or PCR step.

Bosphore SARS-CoV-2/Flu/RSV Panel Kit is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 2019-nCoV (which was later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and Influenza A&B in a single tube from human respiratory samples including nasopharyngeal, oropharyngeal swabs, sputum and saliva. Fluorescence detection uses FAM, HEX, Texas RED, and Cy5 filters. SARS-Cov-2 RNA is amplified, and fluorescence detection is performed using the FAM filter, RSV A&B RNA is amplified, and fluorescence detection is performed using the HEX filter, and Influenza A&B RNA is amplified, and fluorescence detection is performed using the Cy5 filter.  

 An internal control based on detecting human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check RNA extraction, PCR inhibition, sampling, or application errors. Internal control is detected by the Texas RED filter.  

Bosphore SARS-CoV-2/Flu/RSV Panel Kit v2 detects and discriminates 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and influenza A, influenza B in human respiratory samples including nasopharyngeal, oropharyngeal swabs and oral fluids. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.

SARS-CoV-2 is detected by three regions of the virus in a single reaction with Master Mix 1: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the Orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. RSV A&B, influenza A and influenza B are detected and discriminated with Master Mix 2.

Bosphore SARS-CoV-2/Flu/RSV Panel Kit v6 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 2019-nCoV (which is later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and Influenza A&B from human respiratory samples including nasopharyngeal, oropharyngeal swabs, sputum and saliva.  

Fluorescence detection is accomplished using FAM, HEX, Texas RED, Cy5, and Quasar 705 filters.   

 An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed in order to check RNA extraction, PCR inhibition, and sampling or application errors. Internal control is detected by the Texas RED channel.  

Bosphore SARS-CoV-2 / Respiratory Pathogens Panel Kit v1 detects and discriminates 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B , Influenza A&B, Enterovirus, Metapneumovirus, Adenovirus, Human Parainfluenza 1/2/3/4, Rhinovirus, Mycoplasma pneumoniae and Legionella pneumophila, in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva.

Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. SARS-CoV-2 is detected by two regions of the virus (Orf1ab and N gene nCoV specific) from a single channel FAM. RSV A&B is detected by HEX channel. Influenza A&B is detected by Cy5 channel. Enterovirus is detected by FAM channel. Metapneumovirus is detected by HEX channel. Adenovirus is detected by Cy5 channel. Human Parainfluenza virus 1/2/3/4 is detected by FAM channel. Rhinovirus is detected by HEX channel. Legionella pneumophila is detected by Texas Red channel. Mycoplasma pneumoniae is detected by Cy5 channel.

An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. Internal control is detected by Texas Red channel in Tube 1 and Tube 2.

Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v3 Real-Time PCR kit for in vitro diagnostics that detects and characterizes the specified gene regions of the following pathogens from human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.  

 An internal control based on detecting human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check nucleic acid extraction, PCR inhibition, and sampling or application errors. Amplification data of the internal control is detected with the Texas RED filter for PCR Master Mix 2.  

Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v4 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.  

An exogenous internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition, and application mistakes. The amplification data of the exogenous internal control is detected with the Texas RED filter in PCR Master Mix 2. The internal control can be added either during DNA/RNA extraction or the PCR step.  

An endogenous internal control based on the detection with the HEX filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check sampling, extraction, and application errors. The endogenous internal control amplification data is detected with the HEX filter in PCR Master Mix 4.  

Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v5 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, ROX, and Cy5 filters.  

An endogenous internal control based on the detection with the ROX filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check sampling, extraction, PCR inhibition, and application errors. The endogenous internal control amplification data is detected with the ROX filter in PCR Master Mix 4.