Real-Time PCR Kits
Viral Infections in the Immunosuppressed Panel
Immunosuppression is a decrease in the immune system’s activity or effectiveness. Immunosuppression can happen as a negative reaction to the treatment of other illnesses or because of the immunosuppressive effects of some immune system components on other immune system components. Some people who have a weakened immune system, and who are immunocompromised are more likely to get sick for a longer period.
Adenovirus (HAdV), cytomegalovirus (CMV) and Epstein-Barr virus (EBV) are major causes of morbidity and mortality in immunosuppressed individuals. After primary infection, these viruses may remain latent and then occur through immunosuppression. Infection is common with seroprevalence rates increasing steadily from 65% among 40- to 49-year-olds to 91% in those aged 80 years or older.
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Whole blood, serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate, bronchoalveolar lavage |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-( -20°C) / -20°C |
Viral Infections in the Immunosuppressed Panel Kits
Bosphore Viral lnfections in the lmmunosuppressed Panel Kit have been designed to detect Cytomegalovirus (CMV) DNA, Epstein-Barr virus (EBV) DNA and Adenovirus (HAdV) DNA extracted from the following human biological samples; serum, plasma, bone marrow, amniotic fluid, urine, cerebrospinal fluid (CSF), bronchoalveolar lavage (BAL), and whole blood.
An internal control has been integrated into the kit to check PCR inhibition and DNA extraction. The internal control can be added either during DNA extraction or PCR step.
The test is accomplished in a single tube qualitatively, encompassing all the subtypes of CMV, EBV and Adenovirus; CMV DNA (a region within the DNA Polymerase gene) is amplified and fluorescence detection is accomplished using the FAM filter, EBV DNA (a region of IR1) is amplified and fluorescence detection is accomplished using the HEX filter and Adenovirus DNA (a region within the hexon gene) is amplified and fluorescence detection is accomplished using the Cy5 filter. The amplification data of the Internal Control is detected with the Texas Red filter.
| FAM | HEX | Texas RED | Cy5 |
| CMV | EBV | Internal Control | Adenovirus |
Bosphore Viral Infections in the Immunosuppressed Maxi Panel Bundle Kit detects and characterizes JCV DNA, BKV DNA, CMV DNA, EBV DNA and adenovirus DNA human biological samples; serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate, bronchoalveolar lavage. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
| FAM | HEX | Texas Red | Cy5 | |
| PCR Master Mix 1
Box-1 |
JCV | BKV | – | Internal Control |
| PCR Master Mix 1
Box-2 |
CMV | EBV | Internal Control | Adenovirus |
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application errors. Amplification data of the internal control is detected with Cy5 or Texas RED filter as indicated in the table shown above. Internal control can be added either during nucleic acid extraction or PCR step.
Bosphore VIP Quantification Bundle Kit has been designed to detect, discriminate and quantitate cytomegalovirus (CMV), Epstein-Barr virus (EBV/human herpes virus-4/HHV-4) and adenovirus (ADV) DNA in human biological samples including serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate and bronchoalveolar lavage.
Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters in two PCR tubes.
| FAM | HEX | Cy5 | |
| PCR Master Mix 1
Box-1 |
CMV | Internal Control | – |
| PCR Master Mix 1
Box-2 |
Adenovirus (ADV) | Epstein-Barr virus (EBV) | Internal Control |
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step.
Bosphore EBV-Adenovirus Quantification Kit has been designed to detect, discriminate and quantitate Epstein-Barr virus (EBV/human herpes virus-4/HHV-4) and Adenovirus (ADV) DNA in human biological samples including serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate and bronchoalveolar lavage.
Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters in one PCR tube.
| FAM | HEX | Cy5 |
| Adenovirus (ADV) | Epstein-Barr virus (EBV) | Internal Control |
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application errors. The internal control can be added either during DNA extraction or PCR step.
Bosphore JCV-BKV Detection Kit v2 detects and characterizes JC Virus and BK virus in human biological samples. The analytic sensitivity is 175 copies/ml for JCV and 75 copies/ml for BKV. A region within JCV genome is amplified and fluorescence detection is accomplished using the FAM filter. A region within BKV genome is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added either in extraction or PCR step.
